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KMID : 0545119990090020201
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Journal of Microbiology and Biotechnology
1999 Volume.9 No. 2 p.201 ~ p.205
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Effect of Temperature and Carbon Source on the Expression of β-Galactosidase Gene of Lactococcus lactis ssp.lactis ATCC 7962
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Kim, Tae Youn
Lee, Jung Min/Chang, Hae Choon/Chung, Dae Kyun/Lee, Jong Hoon/Kim, Jeong Hwan/Lee, Hyong Joo
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Abstract
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The effects of growth temperature and a carbon source on the expression of β-galactosidase gene of Lactococcus lactis ssp. lactis ATCC 7962 (L. lactis 7962) were investigated. At 25℃, L. lactis 7962 had a higher β-galactosidase activity than cells grown at 30℃ or 37℃. although cells grew most quickly at 37℃. The highest β-galactosidase activity was observed in cells grown in M17 with lactose (1%) followed by cells grown in a galactose (1%) medium. L. lactic 7962 exhibited the minimum β-galactosidase activity in glucose media, indicating catabolite repression. When the cellular levels of β-galactosidase mRNA were examined using slot blot hybridization, no significant differences were observed between cells grown at 25℃ and cells at 30℃ or 37℃ in the same media. This suggests that the quantity of β-galactosidase mRNA may not he the reason for the higher β-galactosidase activities of L. lactis 7962 at 25℃. The level of ccpA (Cataholite Control Protein) transcript remained almost constant during the exponential growth phase irrespective of a carbon source.
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